Effect of Fluctuating Temperature Regime on Psychrophilic Anaerobic Digestion of Nightsoil

  • Lokendra Singh Defence Research & Development Establishment, Gwalior
  • S. I. Alam Defence research & Development Establishment, Gwalior
  • K. V. Ramana Defence research & Development Establishment, Gwalior
Keywords: Hydrogenotrophic methanogens, Volatile fatty acids, Anaerobic digestion

Abstract

The effect of temperature fluctuation on anaerobic digestion of nightsoil using 10 °C adapted inoculum was studied. The digester was subjected to repeated temperature cycling of 10 °C and 30 °C. The total biogas, CH/sub 4/ and H/sub 2/S content, volatile fatty acids and microbial counts were compared with control digesters. No significant deleterious effect was noticed during initial temperature shock of one week. However, repeated exposures reduced the counts of hydrogenotrophic methanogens. There was no effect on the content of CH/sub 4/ and H/sub 2/S in the biogas.

Author Biographies

Lokendra Singh, Defence Research & Development Establishment, Gwalior
Dr Lokendra Singh obtained his PhD (Microbiology) and joined DRDO in 1984. For the last tenyears, he has been working on the biodegradation of organic waste at low temperatures.
S. I. Alam, Defence research & Development Establishment, Gwalior
Mr SI Alam obtained his BSc(Hons) in 1991. He joined DRDO at DRDE, Gwalior, in 1991 asTechnical Assistant. His area of work includes low temperature biodegradation of organic waste.
K. V. Ramana, Defence research & Development Establishment, Gwalior
Dr KV Ramana obtained his MTech (Biotechnology) in 1987. He joined DRDO at DRDE, Gwalior,in 1988. For the last ten years, he has been working on the biodegradation of organic waste at low temperatures.

References

Olsen, J.E. & Larsen, H.Y.E. Bacterial decimation times in anaerobic digestion of animal slurries. Biological Wastes, 1987, 21, 153-68.

Singh, L.; Maurya, M.S.; Sai Ram, M. & Alam, S.I. Biogas production from nightsoil: Effects of loading and temperature. Bioresource Technology,1993,45, 59-61.

Sai Ram, M.; Singh, L. & Alam, S.I. Effect of sulfate and nitrate on anaerobic degradation of nightsoil. Bioresource Technology, 1993, 45, 229-32.

Maurya, M.S.; Singh, L.; Sai Ram. M. & Alam. S.I. Production of biogas from nightsoil: Effect of temperature and volatile solids. Znd. J. Microbiol., 1994,34, 223-28.

Alam, S.I.; Singh, L. & Maurya, M.S. Fatty acid profile during anaerobic digestion of nightsoil: Effect of temperature, calcium carbonate and selectively-enriched inoculum. Def. Sci. J., 1996, 46(1), 21-26.

Singh,L.; Maurya, M.S.; Rarnana, K.V. & Mam, S.I. Production of biogas from nightsoil at psychrophilic temperature. Bioresource Technology, 1995,53, 147-49.

Kalia, A.K. & Kanwar, S. Temperature profiles of biogas plants operating under hilly conditions. Biological Wastes, 1989,30,217-24.

Jain, M.K.; Singh, R. & Tauro, P. Diurnal variation in gas production from biogas plant. Khadigramydog, 198 1,27,314-15.

Hashimoto, S.; Fujita M. & Baccay, R.A. Kinetics of the effect of temperature on the anaerobic digestion of nightsoil. J. Ferment. Technol, 1982, 60, 139-48.

ECDC-TCDC. Renewable sources of energy. Vol 11. United Nations, Economic and Social Commission for Asia and the Pacific, 1981.

Pfenning, N.; Widdel, F. & Truper, H.G. The dissimilatory sulfate reducing bacteria. In The prokaryotes, edited by M.P. Starr, H. Stolp, H.G.Truper, A. Balows & M.P. Schlegel. Springer, Berlin, Germany, 1981, pp. 926-40.

Balch, W.E.; Fox, G.E.; Margrum, C.R.; Woese, C.R. & Wolfe, R.S. Methanogenesis: Re-evaluation of a unique biological group. Microbiology Review, 1979,43,260-96.

Hashimoto, S.; Fujita, M. & Baccay, R.A. Effect of temperature and sludge retention time on the anaerobic digestion of nightsoil. J. Ferment. Technol., 1982,60, 139-48.

McInerney, M.J. Anaerobic hydrolysis and fermentation of fats and proteins. In Biology of anaerobic microorganisms, edited by A.J.B. Zehnder. John Wiley & Sons, New York, 1988.

Widdel, F. Microbiology and ecology of sulfate and sulfur-reducing bacteria. In Biology of anaerobic microorganisms, edited by A.J.B. Zehnder. John Wiley & Sons, New York., 1988.

Published
2013-01-01
How to Cite
SinghL., AlamS., & RamanaK. (2013). Effect of Fluctuating Temperature Regime on Psychrophilic Anaerobic Digestion of Nightsoil. Defence Science Journal, 49(2), 135-140. https://doi.org/10.14429/dsj.49.3798
Section
Biomedical Sciences