Flow-cytometric Analysis of Bacillus anthracis Spores

  • D. V. Kamboj Defence Research & Development Establishment, Gwalior
  • G. S. Agarwal Defence Research & Development Establishment, Gwalior
  • B. S. Dwarakanath Institute of Nuclear Medicine & Allied Sciences, Delhi
  • J. S. Adhikari Institute of Nuclear Medicine & Allied Sciences, Delhi
  • S. I. Alam Defence Research & Development Establishment, Gwalior
  • Lokendra Singh Defence Research & Development Establishment, Gwalior
DOI: https://doi.org/10.14429/dsj.56.1940
Keywords: Flow cytometry, Bacillus anthracis, bacterial spores, FITC, immunofluorescence, bioterrorism, fluorescein isothiocyanate-labelled antibodies, fluorescein isothiocyanate

Abstract

Flow-cytometric technique has been established as a powerful tool for detection and
identification of microbiological agents. Unambiguous and rapid detection of Bacillus anthracis
spores has been reported using immunoglobulin G-fluorescein isothiocyanate conjugate against
live spores. In addition to the high sensitivity, the present technique could differentiate between
spores of closely related species, eg, Bacillus cereus and Bacillus subtilis using fluorescence
intensity. The technique can be used for detection of live as well as inactivated spores making
it more congenial for screening of suspected samples of bioterrorism.
Published
2006-11-01
How to Cite
Kamboj, D., Agarwal, G., Dwarakanath, B., Adhikari, J., Alam, S., & Singh, L. (2006). Flow-cytometric Analysis of Bacillus anthracis Spores. Defence Science Journal, 56(5), 769-774. https://doi.org/10.14429/dsj.56.1940
Issue
Vol 56 No 5
Section
Armaments & Explosives
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