Haematological and Biochemical Changes in Response to Stress Induced by the Administration of Amikacin Injection by Autoinjector in Animals

  • R Vijayaraghavan Saveetha University, Chennai
  • R. Selvaraj Saveetha University, Chennai
  • S. Krishna Mohan Saveetha University, Chennai
  • P. G. Gopi Saveetha University, Chennai
  • C. B. Tharani Saveetha University, Chennai
Keywords: Autoinjector, antibacterial, amikacin, emergency, critical situation.


The drugs administered by autoinjectors, may act fast reducing the morbidity and mortality in critical and emergency situations. Amikacin drug cartridge was developed for the autoinjector as an antibacterial drug for critical situations and its tolerability was studied. Rats were given either 3 doses or 7 doses on consecutive days by the autoinjector (intraperitoneal, 63 mg/mL). Blood was withdrawn on the 4th day (3 doses) or the 8th day (7 doses), and haematological and biochemical parameters were studied. All the parameters studied were within the limits and did not show any significant difference when compared with the control. Rabbits were given 3 doses of two concentrations (intramuscular, 63 or 250 mg/mL) and on the 4th day blood was withdrawn for the haematological and biochemical estimations. 63 mg/mL cartridge did not show any significant change while 250 mg/mL cartridge showed significant change in the haematological and biochemical parameters. This study showed that intraperitoneal injection of amikacin by the autoinjector designed for intramuscular injection was well tolerated by the rats. In the rabbits, low dose (63 mg/mL) was tolerated while the higher dose, which is an adult human dose (250 mg/mL) showed significant changes.

Defence Science Journal, 2014, 64(2), pp. 99-105. DOI: http://dx.doi.org/10.14429/dsj.64.5032

Author Biographies

R Vijayaraghavan, Saveetha University, Chennai

Dr R. Vijayaraghavan obtained his MSc and PhD in Medical Pharmacology from JIPMER, Pondicherry and Jiwaji University, Gwalior, respectively. Presently working as Director-Research at Saveetha University, Chennai. He was awarded with DRDO Agni Award of Excellence in Self Reliance in 2004 and DRDO Titanium Trophy in 2007. He has developed several products, viz., Personal decontamination kit, reusable autoinjectors for nerve gas poisoning, first aid kit for CBW agents, and insect repellent spray and cream. He has about 200 research publications in reputed journals and about 60 patents, copyrights and designs.

R. Selvaraj, Saveetha University, Chennai
Dr R. Selvaraj completed his MPhil and PhD in the pheromone biochemistry and reproductive behavior of rats at Bharathidasan University. He is presently Associate Professor, Centre for Laboratory Animal Technology and Research (CLATR), Sathyabama  University, Chennai. He has published about 25 papers in indexed journals.
S. Krishna Mohan, Saveetha University, Chennai
Dr Surapaneni Krishna Mohan holds PhD (Medical) in Biochemistry and presently working as Associate Professor of Biochemistry and Vice Principal (Admin) in Saveetha Medical College and Hospital, Saveetha University. His research interests include biochemical studies in non alcoholic steatohepatitis and other liver diseases, operations research in population health, public health informatics and research in health professions education.
P. G. Gopi, Saveetha University, Chennai
Dr P.G. Gopi is presently Professor and Head of Statistics, Department of Research, Saveetha University. He Superannuated as Deputy Director, Statistics, from National Institute of Research in Tuberculosis in 2007. He was a WHO consultant for infection survey in India. He is also a member of WHO TB measurement Task Force and visited several countries.
C. B. Tharani, Saveetha University, Chennai
Dr. C.B. Tharani retired as Director Institute of Pharmacology, Madras Medical College. She has guided several PhD and PG students. She was the Principal Investigator for several Phase I Clinical studies. She is the Chairperson of an Independent Ethics Committee.
How to Cite
Vijayaraghavan, R., Selvaraj, R., Mohan, S., Gopi, P., & Tharani, C. (2014). Haematological and Biochemical Changes in Response to Stress Induced by the Administration of Amikacin Injection by Autoinjector in Animals. Defence Science Journal, 64(2), 99-105. https://doi.org/10.14429/dsj.64.5032
Biomedical Sciences