Effect of Topically Applied Sulphur Mustard on Haematological Biochemical and Histological Parameters in Mice

Abstract

Sulphur mustard (SM) a potent blistering agent, has been frequently used as a chemical weapon. In the present study the effect of topically applied SM on haematological biochemical and histological parameters in mice were investigated over a period of seven days. The application of SM resulted in significant increase of blood haemoglobin concentration packed cell volume and erythrocyte count as well as plasma urea uric acid and cholesterol levels. There was a significant decrease in blood leukocyte count Plasma total protein and albumin. There was significant increase in bleeding clotting and prothrombin time. Elevated levels of alkaline phosphatase lactate dehydrogenase aspartate and alaine aminotransaminases and creatine phosophokinase activities were also observed revealing SM-induced liver damage and general state of illness. Histopathological observations revealed a mild to moderate degree of congestion and haemorrhage in the viscera examined. Also there were mild degeneration and ovliteration of chromatin material in liver and Kidneys.