ELISA Detection of Francisella tularensis using Polyclonaland Monoclonal Antibodies

  • Miroslav Pohanka University of Defence, Hradec Kralove
  • Oto Pavlis Central Military Institute of Health, Techonin
  • Michal Kroca Central Military Institute of Health, Techonin
DOI: https://doi.org/10.14429/dsj.58.1693
Keywords: Tularemia, Francisella tularensis, ELISA, biowarfare agent, immunodetection, enzyme-linkedimmunosorbent assay, antibodies, pathogen detection

Abstract

The mouse monoclonal and polyclonal antibodies were produced for the detection of intracellular pathogenand potential warfare agent Francisella tularensis. Antibody titers obtained were 1:640 for polyclonal antibodiesand 1:320 for monoclonal antibodies. Both antibodies were used in the indirect enzyme-linked immunosorbentassay (ELISA) found to detect F. tularensis whole cells. The limit of detection was 5.4×106 CFU/ml for polyclonalantibodies and 6.9×106 CFU/ml for monoclonal antibodies. The value sample could  be distinguished from anyconcentration of another gram-negative bacterium: Escherichia coli.

Defence Science Journal, 2008, 58(5), pp.698-702, DOI:http://dx.doi.org/10.14429/dsj.58.1693

Author Biographies

Miroslav Pohanka, University of Defence, Hradec Kralove
Center of Advanced Studies, Faculty of Military Health Sciences, University of Defence, Hradec Kralove, Czech Republic
Oto Pavlis, Central Military Institute of Health, Techonin
Center of Biological Defense, Central Military Institute of Health, Techonin
Michal Kroca, Central Military Institute of Health, Techonin
Center of Biological Defense, Central Military Institute of Health, Techonin, Czech Republic
Published
2008-09-01
How to Cite
Pohanka, M., Pavlis, O., & Kroca, M. (2008). ELISA Detection of Francisella tularensis using Polyclonaland Monoclonal Antibodies. Defence Science Journal, 58(5), 698-702. https://doi.org/10.14429/dsj.58.1693
Issue
Vol 58 No 5: Nanomaterials: Science & Technology (Part-II)
Section
Research Papers
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