ELISA Detection of Francisella tularensis using Polyclonaland Monoclonal Antibodies

Authors

  • Miroslav Pohanka University of Defence, Hradec Kralove
  • Oto Pavlis Central Military Institute of Health, Techonin
  • Michal Kroca Central Military Institute of Health, Techonin

DOI:

https://doi.org/10.14429/dsj.58.1693

Keywords:

Tularemia, Francisella tularensis, ELISA, biowarfare agent, immunodetection, enzyme-linkedimmunosorbent assay, antibodies, pathogen detection

Abstract

The mouse monoclonal and polyclonal antibodies were produced for the detection of intracellular pathogenand potential warfare agent Francisella tularensis. Antibody titers obtained were 1:640 for polyclonal antibodiesand 1:320 for monoclonal antibodies. Both antibodies were used in the indirect enzyme-linked immunosorbentassay (ELISA) found to detect F. tularensis whole cells. The limit of detection was 5.4×106 CFU/ml for polyclonalantibodies and 6.9×106 CFU/ml for monoclonal antibodies. The value sample could  be distinguished from anyconcentration of another gram-negative bacterium: Escherichia coli.

Defence Science Journal, 2008, 58(5), pp.698-702, DOI:http://dx.doi.org/10.14429/dsj.58.1693

Author Biographies

Miroslav Pohanka, University of Defence, Hradec Kralove

Center of Advanced Studies, Faculty of Military Health Sciences, University of Defence, Hradec Kralove, Czech Republic

Oto Pavlis, Central Military Institute of Health, Techonin

Center of Biological Defense, Central Military Institute of Health, Techonin

Michal Kroca, Central Military Institute of Health, Techonin

Center of Biological Defense, Central Military Institute of Health, Techonin, Czech Republic

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Published

2008-09-01

How to Cite

Pohanka, M., Pavlis, O., & Kroca, M. (2008). ELISA Detection of Francisella tularensis using Polyclonaland Monoclonal Antibodies. Defence Science Journal, 58(5), 698–702. https://doi.org/10.14429/dsj.58.1693

Issue

Vol. 58 No. 5: Nanomaterials: Science & Technology (Part-II)

Section

Research Papers

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