Toxicity Assessment of Biologically Degraded Product of Textile Dye Acid Red G

Priti Faldu; Vishal Kothari; Charmy Kothari; Jalpa Rank; Ankit Hinsu; Ramesh Kothari

doi:10.14429/dlsj.4.14972

Priti Faldu Department of Bioscience, Saurashtra University, Rajkot, India
Vishal Kothari Department of Bioscience, Saurashtra University, Rajkot, India
Charmy Kothari Department of Biotechnology, Christ College, Rajkot, India
Jalpa Rank Department of Biosciences, Saurashtra University, Rajkot
Ankit Hinsu Department of Bioscience, Saurashtra University, Rajkot, India
Ramesh Kothari Department of Bioscience, Saurashtra University, Rajkot, India

DOI: https://doi.org/10.14429/dlsj.4.14972

Keywords: Azo Dye, MTT Assay, Comet Assay, Chromosomal Abberation Assay, Sister Chromatid, Exchange Assay

Abstract

Azo dyes are of environmental concern due to their recalcitrant nature. Several azo dyes and their decolorized and degraded products exert toxic and mutagenic effects on the flora and fauna. The toxic properties of these azo dyes are due to nature and position of the substitution with respect to the aromatic rings and amino nitrogen atoms. Several studies have thus far been emphasized on biodegradation of azo dye pollutants, though role of their biodegraded product is rarely studied. Given a lack of this understanding, we have analyzed the effects of degraded products of a di-azo textile dye Acid Red G by newly isolated bacterial species, Pseudomonas aeruginosa PFK10 and Brevibacillus choshinensis PFK11. The genotoxicity and cytotoxicity of Acid Red G and their degraded products were tested on HeLa cell line and Human lymphocyte cell, respectively. The data of MTT assay has been shown that activity of degraded products of the Acid Red G were comparable to their parent dye. But chromosome aberration assay and sister chromatid exchange assay did not show any significant changes in chromosomes as compared to positive control mitomicine.

Author Biographies

Priti Faldu, Department of Bioscience, Saurashtra University, Rajkot, India

Dr Priti Faldu obtained her PhD (Biochemistry) from Saurashtra University, Rajkot. Presently, she is Assistant Professor at Department of Soil Science and Agricultural Chemistry, N. M. College of Agriculture, Navsari Agricultural University, Navsari. Her research interests are bioremediation, photochemistry, and nutraceuticals. She has published 7 peer-reviewed articles.

Vishal Kothari, Department of Bioscience, Saurashtra University, Rajkot, India

Dr Vishal Kothari obtained his PhD (Biotechnology) from Saurashtra University, Rajkot. Presently, he is working as Research Associate at Department of Biotechnology, Junagadh Agricultural University, Junagadh. His research interests are Plant Growth Promoting Bacteria. He has published 12 peer-reviewed articles

Charmy Kothari, Department of Biotechnology, Christ College, Rajkot, India

Dr Charmy Kothari obtained her PhD (Microbiology) from Saurashtra University, Rajkot. Presently, she is an Associate Professor at Department of Biotechnology, Christ College, Rajkot. Her research interests are Biodegradation of PAHs and Probiotics. She has published 17 peer-reviewed articles.

Jalpa Rank, Department of Biosciences, Saurashtra University, Rajkot

Dr Jalpa Rank obtained her PhD (Biotechnology) from Saurashtra University, Rajkot. Presently, she is Assistant Professor at Institute of Biotechnology, Department of Biosciences, Saurashtra University, Rajkot. Her research interests are microbial bioremediation. She has published 04 peer-reviewed articles.

Ankit Hinsu, Department of Bioscience, Saurashtra University, Rajkot, India

Mr Ankit Hinsu is currently working as CSIR-SRF and pursuing his PhD (Biotechnology) at Saurashtra University, Rajkot. His research interests are molecular microbiology and metagenomics. He has published 10 peer-reviewed articles.

Ramesh Kothari, Department of Bioscience, Saurashtra University, Rajkot, India

Dr Ramesh Kothari obtained his PhD (Microbiology) from Saurashtra University, Rajkot. Presently he is Professor at Department of Biosciences, Saurashtra University, Rajkot. His research interests are environmental microbiology, bioremediation, and metagenomics. He has completed 5 research projects and has published 45+ peer-reviewed articles, 6 books, and 11 book chapters.