Catalysed-microwave based Pretreatment of Lignocellulosic Biomass of Camelina Sativa L. for Bio-Fuel Production

  • Sanjay Mohan Gupta DIBER, DRDO, Min Of Defence, Govt of India, Goraparao, Po-Arjunpur, Haldwani-263139 (UK)
  • Kamal Kumar Defence Institute of Bio-Energy Research
  • Rakshit Pathak Defence Institute of Bio-Energy Research
  • Sanjai Kumar Dwivedi Defence Institute of Bio-Energy Research
Keywords: Bio-fuel, Camelina sativa straw, Catalysed-microwave pre-treatment, Hydrolysis lignocellulosic biomass, Fossil fuels

Abstract

Lignocellulosic biomasses are promising alternative resource for bio-fuel production. But due to the recalcitrant nature of lignin and hemicellulose, necessitates an efficient pre-treatment process to improve the yield of reducing sugars and maximising the enzymatic hydrolysis efficiency. Catalysed-microwave pre-treatment may be a good alternative as compared to other methods since it can reduce the time and improve the enzymatic activity during hydrolysis. The aim of this study was to evaluate the efficiency of the catalysed-microwave based pre-treatment of lignocellulosic biomass of Camelina sativa straw (CSS) to overcome the recalcitrant nature of cellulosic biomass. The microwave-alkaline (2 % NaOH) pre-treatment of CSS at 250 W for 10 min yields maximum (~422 mg/g) total soluble sugars (TSS) production during hydrolysis. Likewise, the maximum glucose content (~294 mg/g) was measured in 2 % alkaline-microwave pre-treatment for 10 min at RT. However, slight increase in lignin degradation was observed with the increase in alkaline hydroxide concentration and microwave irradiation exposure time. The maximum degradation in lignin content (~83 %) was measured in 3 % alkaline-microwave pre-treatment for 20 min at RT. Our results suggest that the microwave-alkaline pre-treatment approach may be employed for comprehensive utilisation of CSS biomass of Camelina sativa L. cv. Calena (EC643910) for bio-fuel production.

Author Biographies

Sanjay Mohan Gupta, DIBER, DRDO, Min Of Defence, Govt of India, Goraparao, Po-Arjunpur, Haldwani-263139 (UK)

Dr Sanjay Mohan Gupta, received his PhD (Biochemistry), in 2007 from University of Lucknow, Lucknow. Presently, working as Scientist-D in DIBER, Haldwani. He is involved in investigation of the antimicrobial and antioxidant activity of different leaf, seed extract and seed oil of Camelina sativa and stinging plants against various pathogenic microorganisms. He is also involved in isolation and characterisation of frankia from rhizosphere of high-altitude actinorhizal plants for sustained soil fertility and ecological restoration of strategic border areas. He has been awarded with ‘Laboratory Scientist of the Year-2010’ by DRDO. He received ‘Young Scientist Award’ by Society for Plant Biochemistry & Biotechnology.

Kamal Kumar, Defence Institute of Bio-Energy Research

Dr Kamal Kumar did his PhD (Botany), from Magadh University, Bodh Gaya, in 2015. Presently working as SRF and involved in the abiotic stress tolerance, phytochemical and toxicity studies in plants at DIBER, Haldwani. He has published 12 research papers in peer reviewed national and international journals.

Rakshit Pathak, Defence Institute of Bio-Energy Research

Mr Rakshit Pathak did his MSc (Chemistry) from DSB campus, Kumaun University, Nainital, in 2013. Presently, working as a JRF in Chemistry Department, DIBER, Haldwani on production of the Biofuel from Camelina and Jatropha crop. He has published two research articles in journal.

Sanjai Kumar Dwivedi, Defence Institute of Bio-Energy Research

Dr Sanjai Kumar Dwivedi did his PhD in Horticulture. Presently he is working as Scientist-F and Addl Director, DIBER, Haldwani. He has been doing pioneering research on propagation, genetic diversity, and cultivation and processing of Seabuckthorn. He has been credited with developing, patenting and commercialisation of Seabuckthorn beverage in India. He is recipient of ‘HIS Golden Jublee Students Award’, Fakharuddin Ali Ahmed Award (ICAR), and DRDO Young Scientist Award.

Published
2017-12-15
How to Cite
Gupta, S., Kumar, K., Pathak, R., & Dwivedi, S. (2017). Catalysed-microwave based Pretreatment of Lignocellulosic Biomass of Camelina Sativa L. for Bio-Fuel Production. Defence Life Science Journal, 3(1), 59-63. https://doi.org/10.14429/dlsj.3.11592
Section
Commemorative Issue - DRDO