PCR-based Methods for Identification and Detection of Phytophthora infestans in Infected Leaves of Tomato

  • Haya Khalid DRDO-Defence Institute of Bio-Energy Research, Haldwani – 263 139, India.
  • Atul Grover DRDO-Defence Institute of Bio-Energy Research, Haldwani – 263 139, India.
  • Sanjai K Dwivedi DRDO-Defence Institute of Bio-Energy Research, Haldwani – 263 139, India.
Keywords: Late blight, PCR, Dipstick, sporangia

Abstract

Every year there is a huge amount of loss of tomato crop due to infection of late blight caused by P. infestans. To minimise this loss, it is important to visualise disease infection, so as the control measures can be taken up rapidly. The aim of this study was to develop a schematic protocol in order to examine the disease outbreak. P. infestans were isolated and identified based on morphological characteristics, serological and species-specific PCR assays. Ten samples were processed and examined morphologically and serologically (dipstick). However, on molecular examination only three isolates were confirmed as P. infestans. Four sets of PCR primers i.e., AE-7, O-8, INF, ITS 3 and ITS 4 were validated for accurate detection of P. infestans infection. All the sets of primers gave positive result by giving amplicons of expected size.

Author Biographies

Haya Khalid, DRDO-Defence Institute of Bio-Energy Research, Haldwani – 263 139, India.
Ms Haya Khalid received her BSc from University of Delhi and MSc (Biotechnology) from Jamia Hamdard University and currently pursuing her PhD at DRDO-BU Center for Life Sciences, Coimbatore. Presently working as a SRF in Defence Institute of Bio-Energy Research, Haldwani. Her research work includes molecular genotypic characterisation of P. infestans strains.
Atul Grover, DRDO-Defence Institute of Bio-Energy Research, Haldwani – 263 139, India.
Dr Atul Grover received his PhD (Biotechnology) from Chaudhary Charan Singh University (Meerut). Currently working as a Scientist ‘D’ in the Molecular biology and Genetic Engineering Laboratory in Defence Institute of Bio-Energy Research, Haldwani. His research interests are centered around molecular biology and field deployment of Bio-eneergy solutions.
Sanjai K Dwivedi, DRDO-Defence Institute of Bio-Energy Research, Haldwani – 263 139, India.
Dr Sanjai K. Dwivedi has received PhD from GB Pant University and currently working as a Scientist ‘F’ in Defence Institute of Bio-Energy Research, Haldwani. His area of research includes utilisation of indigenous plant resources for developing value added products.
Published
2017-12-15
How to Cite
Khalid, H., Grover, A., & Dwivedi, S. (2017). PCR-based Methods for Identification and Detection of Phytophthora infestans in Infected Leaves of Tomato. Defence Life Science Journal, 3(1), 41-44. https://doi.org/10.14429/dlsj.3.11491
Section
Commemorative Issue - DRDO