PCR-based Methods for Identification and Detection of Phytophthora infestans in Infected Leaves of Tomato
Keywords:
Late blight, PCR, Dipstick, sporangia
Abstract
Every year there is a huge amount of loss of tomato crop due to infection of late blight caused by P. infestans. To minimise this loss, it is important to visualise disease infection, so as the control measures can be taken up rapidly. The aim of this study was to develop a schematic protocol in order to examine the disease outbreak. P. infestans were isolated and identified based on morphological characteristics, serological and species-specific PCR assays. Ten samples were processed and examined morphologically and serologically (dipstick). However, on molecular examination only three isolates were confirmed as P. infestans. Four sets of PCR primers i.e., AE-7, O-8, INF, ITS 3 and ITS 4 were validated for accurate detection of P. infestans infection. All the sets of primers gave positive result by giving amplicons of expected size.
Published
2017-12-15
How to Cite
Khalid, H., Grover, A., & Dwivedi, S. (2017). PCR-based Methods for Identification and Detection of Phytophthora infestans in Infected Leaves of Tomato. Defence Life Science Journal, 3(1), 41-44. https://doi.org/10.14429/dlsj.3.11491
Section
Commemorative Issue - DRDO
where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India