Rapid production of therapeutic proteins using plant system

Sathish kumar Ramalingam; G. Iyappan; S. Hari Priya; K. Kadirvelu; J. Kingston; Natarajan Gopalan; R. K. Sharma

doi:10.14429/dlsj.2.11372

Sathish kumar Ramalingam DRDO-BU Centre for Life Sciences, Bharathiar University, Coimbatore – 641 046, India
G. Iyappan DRDO-BU Centre for Life Sciences, Bharathiar University, Coimbatore – 641 046, India
S. Hari Priya Plant Genetic Engineering Laboratory, Department of Biotechnology, Bharathiar University, Coimbatore – 641 046, India
K. Kadirvelu DRDO-BU Centre for Life Sciences, Bharathiar University, Coimbatore – 641 046, India
J. Kingston Defence Food Research Laboratory, Mysuru – 570 011, India
Natarajan Gopalan Defence Food Research Laboratory, Mysuru – 570 011, India
R. K. Sharma Defence Food Research Laboratory, Mysuru – 570 011, India

DOI: https://doi.org/10.14429/dlsj.2.11372

Keywords: Plant biopharming, Plant molecular farming, Agroinfiltration

Abstract

Plant molecular farming is simply defined as the production of proteins therapeutics (PT) in plants, which involves transient gene expression in plants and purification of expressed protein to a great scale for diagnosis, treatment and other applications. This is therapid,economical, safe and reproducible approach for the production of PTas compared to bacterial and mammalian systems. Protein yield and post-translational modifications are the major roadblocks that can be overcome byhigh expression strategies includes over expression constructs, suitable plant host systems and glycoengineering of proteins. The inherent ability of ideally producing safe, functional protein is the most striking phenomenon recognized by the pharmaceutical industries and developed many therapeutic products within few weeks to meet escalating demands during pandemic/epidemic outbreaks recently

Author Biographies

Sathish kumar Ramalingam, DRDO-BU Centre for Life Sciences, Bharathiar University, Coimbatore – 641 046, India

Mr R. Sathishkumar received his MSc, and MPhil, in Botany from Madras Christian College/Madras University and Pondicherry Central University, respectively, and PhD (Biological Sciences) from Madurai Kamaraj University. Currently working as an Associate Professor in the Plant Genetic Engineering Laboratory in Department of Biotechnology. DNA barcoding, Plant Molecular Pharming and Metabolic Engineering are the major thrust areas of the laboratory.
Contributed in over all planing the experiment and editing the paper.

G. Iyappan, DRDO-BU Centre for Life Sciences, Bharathiar University, Coimbatore – 641 046, India

Mr I. Gowtham received the BSc, MSc, and MPhil, in Biotechnology from Periyar University, Bharathiar University and Bharathidasan University, respectively. Currently, he is working as a JRF and pursuing his PhD at DRDO-BU Center for Life Sciences, Coimbatore.
Contributed in doing experiments, compiling the data and writing the paper.

S. Hari Priya, Plant Genetic Engineering Laboratory, Department of Biotechnology, Bharathiar University, Coimbatore – 641 046, India

Ms S. Hari Priya received her BSc from Bharathiar University and completed MSc from, Department of Biotechnology, Bharathiar University.
Contributed in doing experiments, compiling the data.

K. Kadirvelu, DRDO-BU Centre for Life Sciences, Bharathiar University, Coimbatore – 641 046, India

Dr K. Kadirvelu received his MSc, MPhil, and PhD in Environmental Science from Bharathiar University. He has more than 100 publications in Journals. Currently, he is leading DRDO-BU Center for Life Sciences, Bharathiar University as a DRDO co-coordinator.
Contributed in editing the paper

J. Kingston, Defence Food Research Laboratory, Mysuru – 570 011, India

Dr J. Kingston received his PhD in Microbiology from IARI, PUSA. He was awarded with ICAR and CSIR fellowships for the M. Sc and Ph. D respectively. He completed his post doctoral studies in University of Delaware, Newark, Delaware with BOYSCAST fellowship of DST, India. He has made significant contribution in molecular diagnostics of pathogens and in developing vaccines by structural vaccinolgy approach.
Contributed in writing and editing the paper.

Natarajan Gopalan, Defence Food Research Laboratory, Mysuru – 570 011, India

Dr Natarajan Gopalan received his PhD (Zoology) and PDF from OUHSC, USA. Presently working as Scientist ‘F’ at DFRL, Mysuru. He is having vast experience in vector borne diseases diagnosis and monitoring, new process preparation of large scale recombinant proteins for therapeutic and diagnosis purposes and novel therapeutic paradigms to inflammation for digestive diseases like colon and pancreatic cancers.
Contributed in editing the paper.

R. K. Sharma, Defence Food Research Laboratory, Mysuru – 570 011, India

Dr R.K. Sharma received his MPharm (Pharmaceutical Chemistry) from Panjab University and PhD from University of Delhi. Currently working as Director, Defence Food Research Laboratory (DFRL), Mysuru. He has made significant contributions in new drugs, novel drugs delivery systems, herbal radioprotectors, herbal biothreat mitigators and nutraceuticals.
Contributed in editing and the paper