Limitations and Practical Problems in Enzyme Linked Immunosorbent Assays .

  • G.P. Rai Defence Research & Development Establishment, Gwalior
  • K.S. Venkateswaran Defence Research & Development Establishment, Gwalior
DOI: https://doi.org/10.14429/dsj.42.4353
Keywords: ELISA, Enzyme immuno assays

Abstract

The major factors that limit the performance of enzyme-linked immunosorbent assays (ELISAs) have been described. Different variations have been explored for specific applications. Some of the common problems that are encountered while carrying out ELISAs have been highlighted. Suggestions have been made to overcome the difficulties and to improve the reliability of the enzyme immuno assays.

Author Biographies

G.P. Rai, Defence Research & Development Establishment, Gwalior
Dr GP Rai obtained his PhD from Kanpur University in 1981.and joined DRDE, Gwalior, in the same year . His current research interests include microbiology, parasistology, and immunology.
K.S. Venkateswaran, Defence Research & Development Establishment, Gwalior
Mr KS Venkateswaran obtained his MSc in Biochemistry from the University of Madras and is at present working as Scientist C at DRDE, Gwalior. He is one of the recipient in 1985 of DRDO Cash Award for his outstanding contribution' in toxicology'. His current research interests include development of rapid diagnostic tests for the detection of infectious diseases.
Published
2013-01-01
How to Cite
Rai, G., & Venkateswaran, K. (2013). Limitations and Practical Problems in Enzyme Linked Immunosorbent Assays . Defence Science Journal, 42(2), 71-84. https://doi.org/10.14429/dsj.42.4353
Issue
Vol 42 No 2
Section
Biomedical Sciences
Copyright (c) 2016 Defence Science Journal

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