Phytochemical Evaluation and GC-MS Analysis of Leaf Extract of Manihot Esculenta Crantz

Abstract

Plants contain a variety of bioactive compounds which makes them an important foundation of pharmaceutical research. Manihot esculenta Crantz is a perennial shrub with medicinal prospects. The current research was conducted to qualitatively estimate the phytochemicals and Gas Chromatography-Mass Spectroscopy analysis of its methanol extract to identify the compounds present. The qualitative phytochemical estimation was done using standard tests. The GC-MS study of the methanolic extract was done following standard protocol. The phytochemical analysis detected alkaloids, flavonoids, saponins, phenols, steroids, tannins, carbohydrates, glycosides, triterpenoids, ketones, and proteins. A total of 24 bioactive compounds were revealed through GC-MS analysis, including hentriacontane, tetrapentacontane, 1,54-dibromo-, dotriacontyl isobutyl ether, 3-methyl-2-(2 oxopropyl) furan, 4-dodecene-6,8,10-triyn-3-one, (e)-, z,z-6,27-hexatriactontadien-2-one, cholest-5-en-3-ol (3.beta.)-, and propanoate. The findings of this study support its traditional use in management of diabetes. Nevertheless, more research is needed to determine the specific mechanism of action and to characterise the active chemicals found in the extract.