Microbial L Asparaginases Therapeutic and Industrial Applications

Abstract

L-asparaginase (EC 3.5.1.1) is an enzyme that mostly helps break down asparagine into L-aspartic acid and ammonium in water. This enzyme can be found in many living things, like bacteria, plants, and some animals, like the serum of some rodents. Especially for Acute Lymphoblastic Leukaemia (ALL) and Hodgkin’s lymphoma, it is an important chemotherapeutic drug for treating lymphoproliferative diseases and lymphomas. When L-asparaginase comes in contact with water, it breaks down more easily. At the moment, biotechnological methods using certain microorganisms are mostly used to make L-asparaginase. Still, industrial manufacturing needs a study that focuses on both increasing production yields and coming up with new ways to do things, like using different microbes to make enzymes useful in more situations. This review gives an overview of L-asparaginase’s uses and talks about how it is made by different microbes, as well as its limitations, current research, and issues that need to be fixed before it can be used in industry.