In Vivo Antidiabetic Activity and GCMS Analysis of Ethanolic Extracts of Rabdosia Rugosa (Wal ex Benth) H Hara

Monika Mehta; Richa Puri; Geeta Devi; Dechan Angmo; Pooja Boora; Sushila Rani

doi:10.14429/dlsj.8.18893

Monika Mehta Department of Botany, Panjab University, Chandigarh–160 014, India https://orcid.org/0000-0001-6418-1507
Richa Puri Department of Botany, Panjab University, Chandigarh–160 014, India https://orcid.org/0000-0002-4057-0759
Geeta Devi Department of Botany, Panjab University, Chandigarh–160 014, India https://orcid.org/0000-0002-4092-4668
Dechan Angmo Department of Botany, Panjab University, Chandigarh–160 014, India https://orcid.org/0000-0001-6653-2702
Pooja Boora Department of Botany, Panjab University, Chandigarh–160 014, India https://orcid.org/0000-0002-3954-1109
Sushila Rani Department of Botany, Panjab University, Chandigarh–160 014, India https://orcid.org/0000-0002-3086-1157

DOI: https://doi.org/10.14429/dlsj.8.18893

Keywords: Anti-diabetic, Rabdosia rugosa, Alloxan monohydrate, GCMS, FTIR

Abstract

The current study examined the effects of an ethanolic extract of R. rugosa (Rabdosia rugosa (Wall. ex Benth.)
H. Hara) on alloxan-induced diabetes rats, detailing its hypoglycemic potential and using gas chromatography-mass spectrometry to analyze its phytoconstituents. The FTIR spectrum verified the presence of various functional groups in the active ingredients. This research opted to use an extract from fresh leaves and flowers. Chichiri is the local name for the shrub R. rugosa, which belongs to the sage family (Labiatae) and is used as traditional medicine. To determine the hypoglycemic activity of plant extracts the rats were randomly assigned to one of four treatment groups for two weeks of experiments: a normal control group that received no treatment, a diabetic control group that received only alloxan (150 mg/kg BW), a drug control group that received glibenclamide (5mg/kg BW), and a treatment group that received R. rugosa extract (50 mg/kg BW). Our results demonstrated that the extract and medication group saw statistically significant improvement (p≤0.001) in body weight, blood glucose levels, lipid profile, liver and renal parameters. The GCMS analysis showed that numerous active phytoconstituents were present. Phenols, alkanes, alcohols, and other compounds were detected in the FTIR spectrum. After examining the data, we determined that the leaves and inflorescences of R. rugosa have hypoglycemic potential. Continued study of the naturally separated chemicals can aid in the creation of organic medications for diabetic treatment.