Anticancer Activity of Hedychium Coronarium Rhizome Solvent Extracts on Colon Cancer Cells

P. Vishnu Mohan Reddy; Pulala Raghuveer Yadav; K. Venkata Ratnam

doi:10.14429/dlsj.9.18846

P. Vishnu Mohan Reddy Department of Botany, Rayalaseema University, Kurnool–518 007, India https://orcid.org/0000-0002-8677-1345
Pulala Raghuveer Yadav Department of Biotechnology, Indian Institute of Technology Hyderabad, Kandi–502 284, India https://orcid.org/0000-0001-6889-7414
K. Venkata Ratnam Department of Botany, Rayalaseema University, Kurnool–518 007, Indi https://orcid.org/0000-0002-8677-1345

DOI: https://doi.org/10.14429/dlsj.9.18846

Keywords: Anticancer, HCT-116 cells, Hedychium coronarium, Rhizome

Abstract

Hedychium coronarium J. Koenig. is a rhizomatous herb of family Zingiberaceae. It is well known as white ginger lilly or butterfly lilly. The rhizome has been used to treat rheumatism, as a tonic and to avoid bad breath. The present study aim is to evaluate the anticancer, antioxidant and antibacterial activity of petroleum ether (HCPE) and ethanol (HCAE) extracts of Hedychium coronarium rhizome using in vitro methods. Anticancer activity of the extracts was assessed against human colon cancer cell lines (HCT-116). Antioxidant capacity of the HCPE and HCAE was assessed using phosphomolybdenum, DPPH and hydroxyl radical scavenging methods. In results, both HCPE and HCAE extracts have a good amount of total phenolics and flavonoids content. Ethanol extract showed a higher total antioxidant capacity than petroleum ether extract. Petroleum ether extract strongly reduced DPPH purple color, while ethanol extract significantly inhibited hydroxyl radicals. Cytotoxic study results revealed that, the extracts strongly suppressed HCT-116 cell lines in concentration dependent fashion. ELISA and TUNEL assay results showed that petroleum ether extracts enhanced caspase-3 mediated DNA fragmentation in HCT – 116 cell lines. In conclusion, H. coronarium could be used as a source of plant based antibacterial/antioxidant and anticancer components. Further molecular studies are required to isolate and characterize active principle and to validate its pharmacological properties in in vivo models.