A Method for Determination of Protein Concentration in a Given Unknown Sample Using Absorbance Difference Between 205 nm and 280 nm

Abstract

Nowadays, determining the protein content of a sample is a common experiment that is conducted in laboratories. Although there are several ways to measure protein content, dye-based spectrophotometric methods are most frequently used in laboratories. In dye-based approaches, protein assays are mostly carried out at a certain wavelength. Protein concentration tests like Lowry’s take a long time; whereas Bradford’s is quick but requires expensive chemicals. In order to reduce the usage of time and money associated with protein assay, we first looked into and then proposed an easy, affordable, and more accurate technique of determining protein concentration that uses standard curves but doesn’t use any dyes. The difference of two UV wavelength absorbance values at 205 nm and 280 nm was used to determine the protein concentration where one absorbance was recorded for the presence of peptide bonds and another for aromatic proteins. The proposed method has many advantages as it consumes minimum time and chemicals but the major setback is anionic detergents, which can shift the absorbance spectra abruptly.