Evaluation of DNA Extraction Methods of Mule Dung

Rajesh Kumar Vaid; Taruna Anand; Priyanka Batra; Ram Avtar Legha; Bhupendra Nath Tripathi

doi:10.14429/dlsj.4.14269

Rajesh Kumar Vaid National Centre for Veterinary Type Cultures, ICAR-National Research Centre on Equines, Hisar- 125 001, India
Taruna Anand National Centre for Veterinary Type Cultures, ICAR-National Research Centre on Equines, Hisar- 125 001, India
Priyanka Batra National Centre for Veterinary Type Cultures, ICAR-National Research Centre on Equines, Hisar- 125 001, India
Ram Avtar Legha National Research Centre on Equines, Equine Production Campus, Bikaner- 334 001, India
Bhupendra Nath Tripathi National Centre for Veterinary Type Cultures, ICAR-National Research Centre on Equines, Hisar- 125 001, India

DOI: https://doi.org/10.14429/dlsj.4.14269

Keywords: DNA extraction; Kit; Dung; Metagenomics; Mule.

Abstract

DNA isolation is a critical step in microbial community analysis of animal dung. DNA isolation from mule dung is challenging due to microbial diversity, composition and chemical nature of mule dung. Therefore, selection of an appropriate DNA isolation method is important to analyse the complete microbial diversity. In the current study, we evaluated the DNA isolation from mule dung samples (n=11) using QiAmp Mini stool kit as per manufacturer’s procedure with modifications. The results suggest that modifications in proprietary column based method improved the DNA quality and quantity suitable for mule dung microbial community analyses.

Author Biographies

Rajesh Kumar Vaid, National Centre for Veterinary Type Cultures, ICAR-National Research Centre on Equines, Hisar- 125 001, India

Dr R.K. Vaid, received Ph.D in Veterinary Public Health from G.B. Pant University of Agriculture and Technology, Pantnagar, Uttarakhand. He is Pr. Scientist, ICAR-NRCE. His area of research interests includes exploration and capturing of animal microbial biodiversity and taxonomy of veterinary bacteriology, their phenotypic and genotypic characterisation & identification.

Taruna Anand, National Centre for Veterinary Type Cultures, ICAR-National Research Centre on Equines, Hisar- 125 001, India

Dr Taruna Anand, received her Ph.D in Animal Biotechnology from ICAR-National Dairy Research Institute, Karnal. She is currently working as Scientist at National Centre for Veterinary Type Cultures, Hisar and pursuing research on Bacteriophages. She has 40 research article to her credit. She is also the recipient of Lal Bahadur Shastri Young Scientist Award, 2016.

Priyanka Batra, National Centre for Veterinary Type Cultures, ICAR-National Research Centre on Equines, Hisar- 125 001, India

Dr Priyanka Batra, obtained her Ph.D in (Microbiology) from CCS HAU, Hisar, Haryana where she worked on Isolation and evaluation of nodule endophytes for growth promotion in chickpea. Recently, she was employed at ICAR-National Research Centre on Equines, Hisar in Bacteriology laboratory where she worked on molecular characterisation of veterinary microbes

Ram Avtar Legha, National Research Centre on Equines, Equine Production Campus, Bikaner- 334 001, India

Dr R.A. Legha, Ph.D in Livestock Production & Management. He is Pr. Scientist, ICAR-NRCE, presently working on research area dealing with utilisation of animal energy with enhanced system efficiency at equine production campus, Bikaner. His work mainly deals with work stress studies on equines under arid conditions, development and evaluation of donkey drawn agricultural implements, and survey of draught equine population and their utilisation in agricultural operations including transportation.

Bhupendra Nath Tripathi, National Centre for Veterinary Type Cultures, ICAR-National Research Centre on Equines, Hisar- 125 001, India

Dr. B.N. Tripathi, Director, ICAR-National Research Centre on Equines, Hisar. He is Post-doc from BBSRC, UK and Diplomate, ICVP. His major interest areas include: Molecular pathogenesis of paratuberculosis, PPR, equine influenza, glanders, EIA and bacteriophages. He has many awards to his credit.