Application of Enriched Fraction of Seabuckthorn Leaf Extract as Antimicrobial Finish on Technical Textile

M.S. Yogendra Kumar; T. S. Raghu; F. V. Varghese; T. M. Kotresh

doi:10.14429/dlsj.2.12273

M.S. Yogendra Kumar Defence Bioengineering and Electromedical Laboratory, Bengaluru – 560 093, India
T. S. Raghu Defence Bioengineering and Electromedical Laboratory, Bengaluru – 560 093, India
F. V. Varghese Defence Bioengineering and Electromedical Laboratory, Bengaluru – 560 093, India
T. M. Kotresh Defence Bioengineering and Electromedical Laboratory, Bengaluru – 560 093, India

DOI: https://doi.org/10.14429/dlsj.2.12273

Keywords: Antimicrobial activity, Flavonoids, Seabuckthorn, Nomex IIIA.

Abstract

Flavonoid-rich fraction (FRF) from Seabuckthorn leaves extract was prepared by acid hydrolysis process. Total flavonoid content of Seabuckthorn leaves extract and FRF estimated as rutin equivalent was found to be 116.98±3.06 and 277.14 ± 6.78 mg/g of extract/FRF respectively. Its major constituents myrcetin, quercetin, kaempferol and isorhamnetin, were determined by reverse phase high performance liquid chromatography (RP-HPLC). Aramid (NomexIIIA) fabric was treated with triethylene tetramine to increase the wicking height of the fabric for better uptake of FRF. Then, FRF was coated using citric acid as cross linking agent on to aramid fabric by pad-dry-cure method for improved wash durability. FRF coated fabric was characterised using Universal attenuated total internal reflection Fourier Transform Infrared spectroscopy. Effect of FRF coating on flammability property of coated fabric was estimated using flammability tester. There was no significant difference in the char length of the FRF coated fabric and control samples.

Antimicrobial activity of the FRF coated fabric was assessed by both qualitative (agar diffusion method; AATCC 147-2001) and quantitative (percentage reduction test; (AATCC 100-2001) methods using test organisms. The zone of inhibition by agar diffusion method for E. coli and S. aureus was found to be 12.4 mm and 16.7 mm respectively. Quantitative assessment by percentage reduction test showed a reduction percentage of 96.00% and 93.00% for S. aureus and E. coli, respectively. The results of the above study indicate FRF as a valuable ingredient for the development of antimicrobial textiles.

Author Biographies

M.S. Yogendra Kumar, Defence Bioengineering and Electromedical Laboratory, Bengaluru – 560 093, India

Dr Yogendra Kumar M.S. has completed his PhD in chemistry and presently working as scientist D at DEBEL, Bengaluru. Published more than 20 research papers in the peer reviewed journals in the area of chemistry, biochemistry, natural products and technical textiles.
Areas of interests are nanotechnology and technical textiles.

T. S. Raghu, Defence Bioengineering and Electromedical Laboratory, Bengaluru – 560 093, India

Mr T.S. Raghu, has completed MTech in textile engineering persuing his PhD in the field of flame/fire retardant high energy materials. and presently working as Senior Research Fellow at DEBEL, Bengaluru. Area of interest is on flame/fire retardant high energy materials.

F. V. Varghese, Defence Bioengineering and Electromedical Laboratory, Bengaluru – 560 093, India

Dr Francis V. Varghese, has completed Ph D from IIT Delhi and
presently working as scientist F and heading the Texile chemistry
group at DEBEL, Bengaluru. He has developed the technology
for the preparation of the activated carbon spheres and the NBC
protective clothing. Published many research articles in the peer
reviewed journal. Areas of interests are adsorption, carbon, NBC,
Nanotechnology and Technical textiles.

T. M. Kotresh, Defence Bioengineering and Electromedical Laboratory, Bengaluru – 560 093, India

Dr T.M. Kotresh, has completed Ph D from IIT Delhi and presently working as scientist G and Associate Director of DEBEL, Bengaluru. He has developed FRO suit, Anti-G Suit, NBC protective clothing etc. Published more than 25 research articles in the peer reviewed journal
and also some patents. Areas of interests are Technical textiles, NBC protective clothing and nanotechnology.