Comparative Real Time EPR Investigation of Natural and Synthetic Antioxidants: As Potential Anticancer Agents and Radio-protectors

Yanka Karamalakova; Galina Nikolova; Rakesh Kumar Sharma; Raj Kumar; Rajesh Arora; Antoaneta Zheleva; Veselina Gadjeva

doi:10.14429/dlsj.2.11676

Yanka Karamalakova Department Chemistry and Biochemistry, Medical Faculty, Trakia University, Stara Zagora
Galina Nikolova Department Chemistry and Biochemistry, Medical Faculty, Trakia University, Stara Zagora
Rakesh Kumar Sharma Institute of Nuclear Medicine and Allied Sciences, Delhi
Raj Kumar Institute of Nuclear Medicine and Allied Sciences, Delhi
Rajesh Arora Institute of Nuclear Medicine and Allied Sciences, Delhi
Antoaneta Zheleva Department Chemistry and Biochemistry, Medical Faculty, Trakia University, Stara Zagora
Veselina Gadjeva Department Chemistry and Biochemistry, Medical Faculty, Trakia University, Stara Zagora

DOI: https://doi.org/10.14429/dlsj.2.11676

Keywords: DPPH-activity, EPR, Distribution, Radicals, ROS-productionproperties

Abstract

The purpose of this study was to evaluate and compare the free-radical scavenging activity against DPPH stable radical and protective properties of the natural products SQGD and P. corylifolia and synthetic nitroxyl- free radical containing nitrosoureas SLENU and SLCNUgly against in vivo oxidative toxicity of antitumor drug CCNU. It was found statistically significant increase in the DPPH radical-scavenging capacity of both extracts with increase of radiation. The natural antioxidants were localised mainly in the organs and blood after EPR biodistribution study. All combinations of natural extracts/ synthetics agents exhibited considerably lower levels of Asc. radicals as compared to controls. It should be mentioned that the natural antioxidants possess higher oxidative protection in comparison with the synthetic antioxidants. Considerable decrease in ROS production in livers of mice was found after treatment with SQGD, P. corylifolia and SLENU, alone, compared to controls.In conclusion, because of well-expressed antioxidant activities of natural and synthetic antioxidants they might be used in the combination anticancer chemotherapy for reducing toxicity caused by anticancer drugs and/or low levels radiation therapy.

Author Biographies

Yanka Karamalakova, Department Chemistry and Biochemistry, Medical Faculty, Trakia University, Stara Zagora

Dr Yanka Karamalakova is head assistant professor at Medical Faculty, Stara Zagora, Bulgaria and presently working in EPR spectroscopy Division. She is involved in the development and evaluation of natural antioxidants and foods (in vitro) to support hepato-protective, neuro-protective, anti-ulcer, antifatigue, anti-muscular stiffness and anti-depression properties in experimental animals, in vivo.

Galina Nikolova, Department Chemistry and Biochemistry, Medical Faculty, Trakia University, Stara Zagora

Dr Galina Nikolova is head assistant professor at Medical Faculty, Stara Zagora, Bulgariaand working in EPR spectroscopy Division. She is involved in the development and evaluation of natural antioxidants and foods (in vitro) to support neuroprotective and anti-depression properties in Parkinson models of experimental animals, in vivo.

Rakesh Kumar Sharma, Institute of Nuclear Medicine and Allied Sciences, Delhi

Dr Rakesh Kumar Sharma, received his MPharm (Pharmaceutical Chemistry) from Panjab University and PhD from University of Delhi. He is currently Director, Defence Food Research Laboratory (DFRL), Mysuru. He has made significant contributions in new drugs, novel drugs delivery systems, herbal radioprotectors, herbal biothreatmitigators and nutraceuticals.

Raj Kumar, Institute of Nuclear Medicine and Allied Sciences, Delhi

Dr Raj Kumar, is currently Scientist ‘C’ in Radiation Biotechnology Laboratory, Institute of Nuclear Medicine & Allied Sciences, Delhi. He has made significant contributions in new drugs, herbal radioprotectors.

Rajesh Arora, Institute of Nuclear Medicine and Allied Sciences, Delhi

Dr Rajesh Arora is a Director– Life Sciences (Tech. and Admin.) and is currently holding the position of DirectorLife Sciences (A&T) and Scientist ‘F’, and Chief Controller Research and Development (Life Sciences and International Cooperation), Defence Research and Development Organisation, Government of India. His contributions in the area of novel drug design and development, particularly radiation countermeasure agents of natural origin and augmentation of medicinally useful secondary metabolites using biotechnological interventions have received wide acclaim.

Antoaneta Zheleva, Department Chemistry and Biochemistry, Medical Faculty, Trakia University, Stara Zagora

Dr Antoaneta Zheleva is Professor in ‘Chemistry and Biochemistry’ Department and EPR spectroscopy Division, Medical Faculty, Stara Zagora, Bulgaria. She is involved in the development and evaluation of natural antioxidants (in vitro), nitroxyl labelled drugs, free radicals formation and detection by EPR spectroscopy, in vitro and in vivo.

Veselina Gadjeva, Department Chemistry and Biochemistry, Medical Faculty, Trakia University, Stara Zagora

Dr Veselina Gadjeva is Vice Rector at Trakia University, Bulgaria and Professor in ‘Chemistry and Biochemistry’ Department, Medical Faculty, Stara Zagora. She is involved in the development and evaluation of nitroxyl labelled drugs (in vitro), free radicals formation, oxidative stress damages and detection by EPR spectroscopy, in vitro and in vivo.