Gamma Radiation Induced Intestinal Proteomic Modulation in Mice: A Two Dimensional Electrophoretic Analysis

Sania Bajaj; Abhinav Singh; Bhargab Kalita; M.H. Yashavarddhan; Rajiv Ranjan; Humaira Farooqi; Manju Lata Gupta

doi:10.14429/dlsj.2.11668

Sania Bajaj Division of Radioprotective Drug Development Research, Institute of Nuclear Medicine and Allied Sciences, Delhi
Abhinav Singh Division of Radioprotective Drug Development Research, Institute of Nuclear Medicine and Allied Sciences, Delhi
Bhargab Kalita Division of Radioprotective Drug Development Research, Institute of Nuclear Medicine and Allied Sciences, Delhi
M.H. Yashavarddhan Division of Radioprotective Drug Development Research, Institute of Nuclear Medicine and Allied Sciences, Delhi
Rajiv Ranjan Division of Radioprotective Drug Development Research, Institute of Nuclear Medicine and Allied Sciences, Delhi
Humaira Farooqi Department of Biotechnology, Faculty of Science, Jamia Hamdard, Delhi
Manju Lata Gupta Division of Radioprotective Drug Development Research, Institute of Nuclear Medicine and Allied Sciences, Delhi

DOI: https://doi.org/10.14429/dlsj.2.11668

Keywords: Ionising radiations, GI, Proteomics, Two dimensional electrophoresis, MALDI-TOF MS

Abstract

Exposure to high doses of radiation causes serious injuries in gastrointestinal tract, by affecting biomolecules of the tissue. To demonstrate the modulation of intestinal proteome by ionising radiations, we analysed changes in protein expression in 9 Gy irradiated C57BL/6 mice at 24 h and 72 h by using two dimensional electrophoresis technique. A total of 19 protein spots with statistical significance (fold change>1.5 and P<0.05) were found to be differentially expressed. Of these 07 spots were identified by MALDI-TOF MS and peptide mass fingerprinting techniques which matched with the known proteins documented in the online database. These proteins belong to biological-functional categories like cytoskeleton system, molecular chaperones, DNA damage response, and stress response. These identified radiation induced proteins can help in understanding the mechanisms behind the intestinal injuries and thus can become potential targets for therapeutics and also aid in drug development.

Author Biographies

Sania Bajaj, Division of Radioprotective Drug Development Research, Institute of Nuclear Medicine and Allied Sciences, Delhi

Mrs Sania Bajaj received her MSc (Biotechnology) from Amity University, Noida, India and pursuing her PhD Biotechnology) from Jamia Hamdard University, Delhi, India. She is currently working as Research Scholar in INMAS, Delhi, India Her main area of research includes effect of ionizing radiation on the radiosensitive proteins of gastro intestinal (GI) tissue and their modulation in mice by P. hexandrum active principles.

Abhinav Singh, Division of Radioprotective Drug Development Research, Institute of Nuclear Medicine and Allied Sciences, Delhi

Mr Abhinav Singh received his MSc (Zoology) from Allahabad University, Uttar Pradesh, India. He is currently working as Research Scholar in INMAS, Delhi, India. His main area of research includes ionizing radiation reduced oxidative stress and apoptosis pathway in bone marrow and spleen tissue and their modulation in mice by P. hexandrum active principles.

Bhargab Kalita, Division of Radioprotective Drug Development Research, Institute of Nuclear Medicine and Allied Sciences, Delhi

Mr Bhargab Kalita received his MSc (Biotechnology) from Pondicherry University, Puducherry. He is currently working as Research Scholar in INMAS, DRDO, New Delhi, India. His main area of research includes ionising radiation induced inflammatory and cell death pathway response in gastrointestinal tract (GI) and their modulation in mice by P. hexandrum active principles.

M.H. Yashavarddhan, Division of Radioprotective Drug Development Research, Institute of Nuclear Medicine and Allied Sciences, Delhi

Mr MH Yashavarddhan received his MSc (Biotechnology) from KIIT University, Bhuvneshwar, Odisha, India. He is currently working as Research Scholar in INMAS, Delhi, India. His main area of research includes : Radiation induced DNA damage signalling pathway and their modulation in mice by P. hexandrum active principles.

Rajiv Ranjan, Division of Radioprotective Drug Development Research, Institute of Nuclear Medicine and Allied Sciences, Delhi

Mr Rajiv Ranjan received his MSc (Toxicology) from Jamia hamdard University, New Delhi, India. He is currently working as Research Scholar in INMAS, Delhi, India. His main area of research includes : Radiation induced bone marrow suppression and its modulation in mice by P. hexandrum active principles.

Humaira Farooqi, Department of Biotechnology, Faculty of Science, Jamia Hamdard, Delhi

Dr Humaira Farooqi received her PhD (Biochemistry) from Jamia Hamdard University, Delhi, India. She is presently working as Assistant professor, in Jamia Hamdard University, New Delhi, India. She has more than 14 research papers publications in National and international journals. Her main area of research includes : protein biochemistry and stress biology.

Manju Lata Gupta, Division of Radioprotective Drug Development Research, Institute of Nuclear Medicine and Allied Sciences, Delhi

Dr Manju Lata Gupta received her PhD (Zoology) from Chaudary Charan Singh University, Meerut, India. She is presently working as Scientist ‘G’ and Project Director ‘RAKSHAK’ and Head of Division of Radioprotective Drug Development and Research at INMAS, Delhi, India. She has more than 50 research papers publications and 03 patents signed under her. Her area of research interest includes; Radioprotective drug development and expertise in herbal formulations.