Vertical Transmission of Chikungunya virus in Aedes aegypti Mosquitoes from Northern India

  • Anil Kumar Singh Division of Vector Management, Defence Research and Development Establishment, Gwalior
  • Manisha Soni Division of Virology, Defence Research and Development Establishment, Gwalior
  • Ankita Agarwal Division of Virology, Defence Research and Development Establishment, Gwalior
  • Paban Kumar Dash Division of Virology, Defence Research and Development Establishment, Gwalior
  • Manmohan Parida Division of Virology, Defence Research and Development Establishment, Gwalior
  • Natarajan Gopalan Division of Vector Management, Defence Research and Development Establishment, Gwalior
DOI: https://doi.org/10.14429/dlsj.1.10744
Keywords: Chikungunya, mosquito, vertical transmission, trans-ovarial transmission, RT-PCR, genome sequencing

Abstract

Chikungunya virus is now recognised as a resurging arbovirus of global public health significance, with their circulation in both new and old world. It is horizontally transmitted among vertebrates by Aedes mosquitoes. So far, the existence of vertical transmission of Chikungunya virus in Aedes vector is riddled with conflicting reports. In this study, presence of Chikungunya virus was detected in adult Aedes aegypti mosquitoes that emerged from field-collected larvae from Gwalior, northern India during 2010. This was further confirmed through nucleotide sequencing that revealed the presence of novel east central south African (ECSA) genotype of Chikungunya virus. This provides molecular evidence for vertical transmission of Chikungunya virus in mosquitoes in nature, which may have important consequences for viral survival during inter-epidemic period and adverse climatic conditions.

Author Biographies

Anil Kumar Singh, Division of Vector Management, Defence Research and Development Establishment, Gwalior
Dr Anil K Singh has worked as fellow in DRDE, Gwalior and got his PhD from Bharathiyar University. He has extensive laboratory experience in biological chemistry, fermentation and purification, analysis of Pharmaceutical products, instrumental and microbiological and vector biology. He has 15 publications in his credit
Manisha Soni, Division of Virology, Defence Research and Development Establishment, Gwalior
Ms Manisha Soni is student form DRDE, Gwalior working on Vector Biology and Disease monitoring, transmission and its control as well as intro screening of plant products
Ankita Agarwal, Division of Virology, Defence Research and Development Establishment, Gwalior
Dr Ankita Agarwal got her PhD in 2016 from Bharathiyar University. Worked as SRF at DRDE, Gwalior and her major research area is Virology. Ankita has published 07 papers in International journal. Presently she is working as Scientist in ICMR, Bhopal
Paban Kumar Dash, Division of Virology, Defence Research and Development Establishment, Gwalior
Dr Paban K. Dash is currently working as a Senior Scientist at Defence Research & Development Establishment (DRDE), Gwalior. His Masters in Virology from Indian Veterinary Research Institute (IVRI), Mukteshwar and PhD in Microbiology from Jiwaji University, Gwalior. He has published more than 50 articles in International journals and guided 02 PhD students. His PDF from Pasteur Institute, Paris and recipient of many national awards
Manmohan Parida, Division of Virology, Defence Research and Development Establishment, Gwalior
Mr Manmohan Parida is a senior Scientist and Head of Virology division in DRDE, Gwalior as well as fellow of Nagasaki University. He has guided many students recipients national and internationals awards. He has 75 publications and 15 patents in his credit. He developed simple filed test kits for newly emerging viral diseases
Natarajan Gopalan, Division of Vector Management, Defence Research and Development Establishment, Gwalior
Dr Natarajan Gopalan has got his PhD in Zoology and vast experience in Vector borne diseases diagnosis and monitoring, New Process preparation of large scale recombinant proteins for Therapeutic and diagnosis purposes and novel therapeutic paradigms to inflammation for digestive diseases like colon and pancreatic cancers. His PDF from OUHSC, USA. He has 69 publications and 16 patents in his credit. He has guided 04 PhD students. He worked at DRDE, Gwalior and DRDO-BU CLS, Coimbatore at various capacities. Presently working as senior scientist at DFRL, Mysore
Published
2016-10-07
How to Cite
Singh, A., Soni, M., Agarwal, A., Dash, P., Parida, M., & Gopalan, N. (2016). Vertical Transmission of Chikungunya virus in Aedes aegypti Mosquitoes from Northern India. Defence Life Science Journal, 1(2), 184-187. https://doi.org/10.14429/dlsj.1.10744
Issue
Vol 1 No 2 (2016)
Section
Research Article

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