In Vitro Antioxidant and Anti-rhizopus Activity of Methanolic Seed Extract of Camelina Sativa L.

Abstract

The antifungal efficacy of seed extracts (propanol, ethanol, methanol and sterile deionised water) of Camelina sativa cv. Calena (EC643910) against Rhizopus stolonifer was investigated by agar disc diffusion method. Among all extracts, methanolic seed extract (Disc 3) showed significant activity against R. stolonifer. However, no activity was observed against rest of the extracts (Disc 1, 2 and 4). The inhibition zones were ~35 mm for methanolic extract and ~41 mm for standard drug, respectively. The total phenol content was observed 13.5 μmol, 23.3 μmol, 42.9 μmol and 3.4 μmol gDW-1 in propanolic, ethanolic, methanolic and sterile deionised water extracts of Camelina, respectively. Likewise, the ascorbate peroxidase (APX) activity was observed highest in methanolic extract that was ~10.2, ~2.4 and ~5.7-folds higher as compared to propanolic, ethanolic and sterile deionised water extracts, respectively. Similarly, guaiacol peroxidase (GPX) activity was also observed highest in methanolic extract as compared to other extracts, which is ~7.3, ~1.9 and ~6.6-folds higher as compared to propanolic, ethanolic and sterile deionised water extracts, respectively. The findings of the study clearly speculate that the possibility of using methanolic seed extract of Camelina as a potential control measure against R. stolonifer, which may be used for the development of future herbal drug formulations.