Flow-cytometric Analysis of Bacillus anthracis Spores

D. V. Kamboj, G. S. Agarwal, B. S. Dwarakanath, J. S. Adhikari, S. I. Alam, Lokendra Singh

Abstract


Flow-cytometric technique has been established as a powerful tool for detection and
identification of microbiological agents. Unambiguous and rapid detection of Bacillus anthracis
spores has been reported using immunoglobulin G-fluorescein isothiocyanate conjugate against
live spores. In addition to the high sensitivity, the present technique could differentiate between
spores of closely related species, eg, Bacillus cereus and Bacillus subtilis using fluorescence
intensity. The technique can be used for detection of live as well as inactivated spores making
it more congenial for screening of suspected samples of bioterrorism.

Keywords


Flow cytometry, Bacillus anthracis, bacterial spores, FITC, immunofluorescence, bioterrorism, fluorescein isothiocyanate-labelled antibodies, fluorescein isothiocyanate

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DOI: http://dx.doi.org/10.14429/dsj.56.1940



Defence Science Journal (DSJ)