| || Impact of Phospho-ELK-1 Expression in Enterocytes in Biodosimetry after Low-dose Irradiation
Author : Osterreicher, J.;Driak, D.;Vilasova, Z.;Vavrova, J.
Source : Defence Science Journal ; Vol:57(6) ; 2007 ; pp 845-851
Subject : 60 Biotechnology;61 Medical Sciences
Keywords : Radiation;Biodosimetry;Phospho-ELK-1;Image analysis;Immunohistrochemistry;Biomarkers
Abstract : Previously the higher phospho-ELK-1 (p-ELK-1) expression in enterocytes 24-96 h after sub-lethal irradiation was measured. Therefore, purpose of this study is to examine in vivo expression of this common ERK1/2 and JNK/SAPK target during the first hours after low-dose irradiation to assess ability of this transcriptional factor as a biodosimetric marker after the single whole-body irradiation. The laboratory rats were randomly divided into 12 groups and irradiated with whole-body gamma radiation dose of 0.25 Gy, 0.5 Gy, and 1.0 Gy. Samples of jejunum were taken 1 h, 2 h, 4 h, and 24 h later, the p-ELK-1 was immunohistochemically detected and then its expression was measured by computer image analysis. A significantly increased expression of p-ELK-1 in enterocytes was measured 1 h, 2 h, and 4 h after 0.5 Gy and 1.0 Gy irradiation, 24 h after all radiation doses, respectively. Peak of p-ELK-1 expression was observed at 0.5 Gy at all time points after irradiation. The detection of p-ELK-1 might be considered as a perspective qualitative in vivo biodosimetric marker for military purposes expressed by proliferative cells 24 h after irradiation with doses from 0.25 Gy to 1.0 Gy.