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 | Flow-cytometric Analysis of Bacillus anthracis Spores
Author : Kamboj, D. V.;Agarwal, G. S.;Dwarkanath, B. S.;Adhikari, J. S. ;Alam, S. I. ;Singh, Lokendra
Source : Defence Science Journal ; Vol:56(5) ; 2006 ; pp 769-774
Subject : 57.089 Biomedical Sciences
Keywords : Flow cytometry;Bacillus anthracis;Bacterial spores;FITC;Immunofluorescence;Bioterrorism;Fluorescein Isothiocyanate-labelled antibodies;Fluorescein isothiocyanate
Abstract : Flow-cytometric technique has been established as a powerful tool for detection and identification of microbiological agents. Unambiguous and rapid detection of Bacillus anthracis spores has been reported using immunoglobulin G-fluorescein isothiocyanate conjugate against live spores. In addition to the high sensitivity, the present technique could differentiate between spores of closely related species, eg, Bacillus cereus and Bacillus subtilis using fluorescence intensity. The technique can be used for detection of live as well as inactivated spores making it more congenial for screening of suspected samples of bioterrorism. |
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