| || Emulsification and Hydrolysis of Oil by Syncephalastrum racemosum
Author : Mathur, Chandni;Prakash, Ranjana;Ali, Amjad;Kaur, Jasminder;Cameotra, Swaranjit Singh;Prakash, N. Tejo
Source : Defence Science Journal ; Vol:60(3) ; 2010 ; pp 251-254
Subject : 57 Biological Sciences;Defence Science Journal
Keywords : Fungus;Syncephalastrum racemosum;emulsification;esterification;vegetable oil;biotransformation;lipolysis
Abstract : A fungal strain, Syncephalastrum racemosum, was isolated from oil-leak contaminated soils from Kanpur, India. The strain was examined for potential to emulsify soybean oil using a 18 per cent oil supplement as carbon source in minimal salt medium. On 72 h growth of the fungus in oil and salt medium, the cellfree supernatant (CFS) showed presence of mono- and di-glycerides indicating degradation of oils to free fatty acids (FFAs). Increasing concentration of oil resulted in enhanced formation of FFAs. The degradation process was observed to be related to the emulsification activity in CFS which was observed to increase with time. The study reports the emulsification and hydrolytic activity of S. racemosum, an activity that can be exploited for increasing the accessibility and treatment of hazardous substance including hydrophobic explosives.