| || Antioxidant and Radioprotective Effects of Ocimum Flavonoids Orientin and Vicenin in Escherichia coli
Author : Nayak, Vrinda;Nishioka, Hajime ;Uma Devi, P.
Source : Defence Science Journal ; Vol:56(2) ; 2006 ; pp 179-187
Subject : 57 Biological Sciences ;579 MicroBiology
Keywords : Escherichia coli;Escherichia coli mutants DSH19 and DSH56;Orientin;Vicenin;Radioprotection;Antioxidant effect;Ocimum flavonoids;Reactive oxygen species
Abstract : Antioxidant effect of the Ocimum flavonoids, orientin and vicenin (25-500 mM), was evaluated by the kat-sod assay in Escherichia coli mutants (DSH56, superoxide dismutase-deficient and DSH19, catalase-deficient) treated with 50 mM menadione or H2O2 (1mM). Protection by orientin (200 mM) and vicenin (200 mM) against H2O2-induced DNA damage in DSH19 cells (b-galactosidase test) and against radiation lethality in wild-type (DSH7) and DSH19 cells exposed to 0-150 Gy gamma radiation was also studied. Menadione and H2O2 reduced the surviving fraction to 0.2 and 0.4 in DSH56 and DSH19 cells, respectively. Even 25 mM of either flavonoid significantly increased the surviving fraction, with maximum protection at 200 mM. H2O2 increased the b-galactosidase activity in a concentration-dependent manner, which was significantly (P < 0.050–0.001) reduced by orientin and vicenin (200 mM). Radiation produced a dose-dependent decrease in the surviving fraction of both DSH7 and DSH19 cells. Pretreatment with 200 mM orientin or vicenin significantly increased the survival (DRF: DSH7 = 2.2; DSH19 = 1.8). Both compounds were equally effective in reducing the cytotoxicity of radiation and the chemical oxidants. The cytoprotective action of these plant flavonoids could be ascribed to their free radical scavenging activity.
| || High Yield Production of Heterologous Proteins with Escherichia coli
Author : Tripathi, N.K. ;Sathyaseelan, K. ;Jana, A.M. ;Rao, P.V.L.
Source : Defence Science Journal ; Vol:59(2) ; 2009 ; pp 137-146
Subject : 57 Biological Sciences
Keywords : Fermentation;Escherichia coli;Scale up;Media;Yeast extract;High cell density
Abstract : The demand for therapeutic recombinant proteins is set to see a significant increase over the next few years. As a consequence, the processes used to produce these proteins must be able to meet market requirements. Recombinant proteins have gained enormous importance for clinical applications. The present paper reviews the fermentation process for high yield scalable production of heterologous recombinant proteins in Escherichia coli. Influence of process parameters for the standard fermentation processes are discussed, and alternative methods that solve the limitations are reviewed together with the methods that yielded in higher productivity of E. coli process. The common problems are scale-up issues, plasmid instability, acetate accumulation and substrate inhibition in the high cell density bioreactor production system. Methods to overcome these issues are described. Solving the problems makes ideal condition for high yield production with E. coli expression system as compared to other systems.